Fig. 6: Blocking FGF1/FGFR signalling suppresses the proliferation, migration and invasion of NPC cells.

a HONE1 cells were incubated for 4 h of AZD4547 at different concentrations and then lysed and immunoblotted for the indicated proteins. b, c HONE1 cells stably overexpressing LHX2 or the empty vector were treated with AZD4547 (100 nM) or DMSO. Whole-cell lysates were subjected to western blot analysis. Each experiment was independently repeated at least three times. d–f HONE1 cells stably overexpressing LHX2 or the empty vector were treated with AZD4547 (100 nM) or DMSO for 48 h. The cells were harvested and subjected to (d) colony-formation assay, (e) wound-healing assay and (f) transwell migration and invasion assays. Each experiment was independently repeated at least three times. g–j LHX2-overexpressed or vector HONE1 cells (2 × 10⁶) were injected into the dorsal flank of mice. One week after injection, the mice were treated with AZD4547 (12.5 mg/kg/d) or vehicle orally for 3 weeks. Images of Xenograft tumours (g). Tumour volume growth curves (h). The volume of xenograft tumours (i). Average xenograft tumour weights (j). LHX2-overexpressed or vector HONE1 cells (1 × 10⁶) were injected into the tail vein of nude mice and the mice were treated with AZD4547 (12.5 mg/kg/d) or vehicle orally for 3 weeks. k–m Representative images of metastatic lungs (k), representative HE images (l) and numbers of metastatic foci per lung (m) (n = 5 in each group). Data are shown as means ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.