Fig. 1: FAK promotes murine PDAC growth associated with increased stromal expression of PD-L2.
From: FAK promotes stromal PD-L2 expression associated with poor survival in pancreatic cancer
a Representative western blot of Panc47 FAK-wt and FAK−/− whole cell lysates probed with anti-FAK and anti-GAPDH antibodies. b Average weight of Panc47 FAK-wt and FAK−/− tumours 2 and 3 weeks post-implantation of 0.5 × 106 cells into the pancreas of C57BL/6 mice. n = 8 tumours per group. c Kaplan–Meier survival plot of C57BL/6 mice implanted with Panc47 FAK-wt and FAK−/− tumours. n = 8 mice per group. Log-rank (Mantel-Cox) test, p = 0.0005; Gehan-Breslow-Wilcoxon test, p = 0.0015. d Nanostring gene expression analysis of RNA isolated from Panc47 FAK-wt and FAK−/− tumours. n = 3 tumours per group. e t-sne map of flow cytometry data from Panc47 FAK-wt and Panc47 FAK−/− tumours. Data generated from all live cells in a representative tumour. f Flow cytometry quantification of PD-L2 expression in Panc47 FAK-wt and FAK−/− tumours. n = 9 tumours per group. Data represented as mean ± s.e.m. Two-tailed unpaired t-test, ****p ≤ 0.0001, ***p ≤ 0.001, **p ≤ 0.01, *p ≤ 0.05.
