Fig. 5: FAK-dependent expression of IL6 amplifies IL4-dependent PD-L2 expression.
From: FAK promotes stromal PD-L2 expression associated with poor survival in pancreatic cancer
a Quantitative analysis of chemokines / cytokines present in media conditioned by either Panc47 FAK-wt or Panc47 FAK−/− cells for 48 h. b Flow cytometry quantification of PD-L2 expression on bone-marrow derived macrophages cultured in normal growth media (M), M + IL6, M + IL4 and M + IL6 + IL4. Data represented as fold-change in median fluorescence intensity relative of M alone. n = 3 per condition, ordinary one-way ANOVA with Tukey’s multiple comparison. c ELISA quantification of IL6 in media conditioned by either Panc47 FAK-wt or Panc47 FAK−/− cells untreated or stimulated with IL17. n = 3, two-tailed unpaired t-test. d ELISA quantification of IL6 in media conditioned by either Panc47 FAK-wt (n = 7), FAK−/− (n = 7), FAK-wt control shRNA (n = 7), FAK-wt IL6 shRNA1 (n = 6) and FAK-wt IL6 shRNA2 cells (n = 6). One-way ANOVA with Dunnett’s multiple comparison. e Fold-change in the average weight of Panc47 FAK-wt (n = 14), FAK−/− (n = 11), FAK-wt control shRNA (n = 4), FAK-wt IL6 shRNA1 (n = 6) and FAK-wt IL6 shRNA2 (n = 6) tumours 2 weeks post-implantation of 0.5 × 106 cells into the pancreas of C57BL/6 mice. Ordinary one-way ANOVA with Tukey’s multiple comparison. f Flow cytometry quantification of PD-L2 expression in Panc47 FAK-wt CTL shRNA (n = 7), FAK-wt IL6 shRNA1 (n = 3) and FAK-wt IL6 shRNA2 (n = 3) tumours. Ordinary one-way ANOVA with Tukey’s multiple comparison. All data represented as mean ± s.e.m. ****p ≤ 0.0001, ***p ≤ 0.001, **p ≤ 0.01, *p ≤ 0.05.
