Table 1 Measured binding affinities of rHSA and AlproTox variants to hFcRn (KD, Kon and Koff) at pH 5.5.

From: Identification of a high-risk immunogenic prostate cancer patient subset as candidates for T-cell engager immunotherapy and the introduction of a novel albumin-fused anti-CD3 × anti-PSMA bispecific design

 

KD (M)

Kon (1/ms)

Koff (1/s)

R2 1:1 fit

X2 fit

Albumin NB

–

–

–

–

–

Albumin WT

2.1E-07

2.8E + 04

5.8E-03

0.979

0.788

Albumin HB

7.7E-09

2.5E + 04

1.83E-04

0.997

0.865

AlproTox NB

–

–

–

–

–

AlproTox WT

1.1E-08

1.8E + 04

1.96E-04

0.998

0.853

AlproTox HB

5.3E-09

5.0E + 04

2.6E-04

0.9982

0.459

  1. Data were obtained by Bio-Layer Interferometry using an Octet system. Sensors were coated with hFcRn and lowered into wells containing the analyte. For affinity analyses, the sensorgrams were fitted to a 1:1 model.
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