Fig. 1: Adenosine inhibits CAR T cell activation.
From: Impact of the selective A2AR and A2BR dual antagonist AB928/etrumadenant on CAR T cell function
a Schematic illustration of the rationale of combining CAR T cell therapy and AB928. b, c In all, 2.5 × 104 murine anti-EpCAM CAR T cells were cocultured with 2.5 × 104 Panc02-EpCAM tumour cells for 24 h in the presence or absence of b NECA (titration from 0.1 nM to 10 µM) or c adenosine (titration from 1 µM to 100 µM + EHNA 2.5 µM). b IFN-γ, IL-2 and TNF-α concentrations in the coculture supernatant were determined by ELISA. Data were normalised to the vehicle control condition. c IFN-γ concentrations in the coculture supernatant were determined by ELISA. b, c Data are shown as mean ± SEM of n = 3 independent experiments with each dot representing the mean value of an individual experiment. *P < 0.05 by two-sided t test.
