Fig. 2: AB928 protects CAR T cells from adenosine mediated suppression.
From: Impact of the selective A2AR and A2BR dual antagonist AB928/etrumadenant on CAR T cell function
a In all, 2.5 × 104 murine anti-EpCAM CAR T cells were cocultured with 2.5 ×104 Panc02-EpCAM tumour cells for 24 h in the presence or absence of NECA (100 nM) and AB928 (titration from 0.1 nM to 10 µM). IFN-γ, IL-2 and TNF-α concentrations in the coculture supernatant were determined by ELISA. Data were normalised to the vehicle control condition. b Also, 2.5 ×104 murine anti-EpCAM CAR T cells were cocultured with 2.5 × 104 4T1, LL/2-EpCAM, T110299-EpCAM and CT26-EpCAM tumour cells for 24 h cells in the presence or absence of NECA (100 nM) and AB928 (100 nM). Cytokine concentrations in the coculture supernatant were determined by IFN-γ ELISA. c Intracellular staining of murine anti-EpCAM CAR T cells activated with plate bound recombinant EpCAM for 18 h in the presence or absence of NECA (1 µM) and AB928 (1 µM). a–c Data are shown as mean ± SEM of a, c n = 3 or b n = 4–5 independent experiments with each dot representing the mean value of an individual experiment. *P < 0.05, **P < 0.01, ***P < 0.001 by one-way ANOVA.
