Fig. 3: Metformin rescues F. nucleatum-induced stemness by inhibiting sonic hedgehog pathway. | British Journal of Cancer

Fig. 3: Metformin rescues F. nucleatum-induced stemness by inhibiting sonic hedgehog pathway.

From: Metformin abrogates Fusobacterium nucleatum-induced chemoresistance in colorectal cancer by inhibiting miR-361-5p/sonic hedgehog signaling-regulated stemness

Fig. 3

a, b Real-time PCR was performed to evaluate SHH and GLI1 expression in HT-29 (a) and HCT 116 (b) cells co-cultured with F. nucleatum or treated with 1 mM metformin for 24 h; unpaired t test. c SHH and GLI1 levels were detected by western blotting in HT-29 and HCT 116 cells. The cells were co-cultured with F. nucleatum or treated with 1 mM metformin for 24 h. d Western blotting was performed to detect sonic hedgehog pathway-related proteins in xenograft tumors after different treatments. n.s., P > 0.05; ***P < 0.001; ****P < 0.0001.

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