Fig. 4: Effect of RARα KD on cellular apoptosis in MDS-L and MOLM-13 cells. | British Journal of Cancer

Fig. 4: Effect of RARα KD on cellular apoptosis in MDS-L and MOLM-13 cells.

From: All-trans retinoic acid enhances the cytotoxic effect of decitabine on myelodysplastic syndromes and acute myeloid leukaemia by activating the RARα-Nrf2 complex

Fig. 4

a Transcriptional levels of RARα in sh1 and sh2 cells were significantly reduced compared with the shCtrl. Immunoblots with anti‐RARα and anti‐GAPDH antibodies showed that RARα protein expression was markedly decreased in sh1 and sh2 cells. Flow cytometry staining with annexin V-APC (b) and western blotting results (c) showed that RARα KD reversed the apoptosis induced by the combined treatment, but not that induced by DAC alone. MDS-L and MOLM-13 cells were treated with DAC (0.8 and 1.5 μM, respectively) or DAC and ATRA (4 and 2 μM, respectively) for 48 h.

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