Fig. 2: Norepinephrine (NE) inhibits the secretion of CXCL9 by tumour cells, leading to reduced chemotaxis of CD8+ T cells in tumour microenvironment. | British Journal of Cancer

Fig. 2: Norepinephrine (NE) inhibits the secretion of CXCL9 by tumour cells, leading to reduced chemotaxis of CD8+ T cells in tumour microenvironment.

From: Norepinephrine inhibits CD8+ T-cell infiltration and function, inducing anti-PD-1 mAb resistance in lung adenocarcinoma

Fig. 2

a The expression levels of common chemokines and receptors were detected by PCR array; b the expression of CXCL9 in the tumours of CTRL + Vehicle, NE + anti-PD-1 mAb + Vehicle and anti-PD-1 mAb + Vehicle groups was detected by western blotting; c the plasma content of CXCL9 was also lower in the resistance group; d the plasma content of CXCL9 was greatly correlated with the content of NE; the CXCL9 mRNA/protein expression levels in A549 and H3122 cells were detected by RT-qPCR (e), ELISA (f) and western blotting (g); h the quantification of CD8+ T-cell migration activity was measured and compared; i the CXCL9 expression level in tumours was detected in the anti-PD-1 mAb+vehicle and anti-PD-1 mAb+NE + vehicle groups using immunohistochemistry. *P < 0.05, **P < 0.01.

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