Fig. 5: Branched-chain amino acids (BCAAs) increased cell growth and invasion via mTOR signalling in ICC cells.
From: Convergent genomic diversity and novel BCAA metabolism in intrahepatic cholangiocarcinoma
a Western blotting of BCAT1 and BCAT2 in ICC cells. BCAT1, BCAT2, and b-actin protein levels in human ICC cell lines (SSP-25, RBE and HCCC-9810) and normal human dermal fibroblast cells were evaluated using western blotting. b Western blotting of mTOR signalling proteins in ICC (SSP-25 and HCCC-9810) cells. c Cell growth of ICC cells with/without BCAAs. Cell growth of SSP-25 and HCCC-9810 cells was measured using SRB assay. d Cell invasion of ICC cells with/without BCAAs. Cell invasion was measured using SSP-25 and HCCC-9810 cells. e Western blotting of mTOR signalling proteins in BCAT1 and BCAT2 knockdown ICC (SSP-25) cells. f Cell growth of BCAT1 and BCAT2 knockdown ICC (SSP-25) cells. Cell growth of shBCAT1/shBCAT2-expressing SSP-25 cells was measured using SRB assay.
