Fig. 2: Technical performance of the nanoString assay for the 24-gene hypoxia signature. | British Journal of Cancer

Fig. 2: Technical performance of the nanoString assay for the 24-gene hypoxia signature.

From: Technical development and validation of a clinically applicable microenvironment classifier as a biomarker of tumour hypoxia for soft tissue sarcoma

Fig. 2

a The raw (pre-normalisation data) gene expression profiles are shown for a single reference RNA sample in the first run versus each subsequent repeat (n = 12 repeats). Each data point is the expression value for an individual hypoxia gene. Spearman’s ρ > 0.99 for all repeats. The mean versus variance of the raw expression values for each gene (black data points are hypoxia genes, and green data points are endogenous control genes) are plotted for (b). the Manchester cohort; and c the VORTEX-Biobank cohort demonstrating low variance of the endogenous controls compared to hypoxia signature genes. RNA quality control measures are shown for hypoxia-low versus hypoxia-high samples from the combined cohort (n = 280). No statistically significant differences (Mann–Whitney U test) were found in (d). RNA yield; e RNA integrity number (RIN); f DV200 (percentage of RNA fragments over 200 bases) or g sample age.

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