Fig. 2: Immunohistochemical analysis.

a Workflow of digital image analysis using QuPath. b Representative IHC-staining, cell detection and classification. Detected cells were classified and colour-coded using QuPath. Blue, negative tumour cells. Yellow, weakly stained tumour cells. Orange, moderately stained tumour cells. Red, strongly stained tumour cells. Green, cells of the tumour-microenvironment with increasing darkness according to intensity of protein expression. Images on the right are magnifications of areas in the pictures on the left.