Fig. 5: Targeting intrinsic dependency on ser/gly synthesis in combination with RT increases ROS levels in NSCLC cell lines.
From: Targeting serine/glycine metabolism improves radiotherapy response in non-small cell lung cancer
a NSCLC cell lines were pre-treated with sertraline for 48 h, and then irradiated with 6 Gy with and without sertraline. Cellular ROS levels were analyzed 24 h post-RT by flow cytometry using cellROX probe. The graphs represent the fold change of Mean Fluorescence Intensity (MFI) for n = 3 independent experiments. One-way ANOVA with Tukey’s multiple comparison test was used. Data are represented as mean ± standard deviation. Individual dots represent independent observations. Statistical analysis *p-value < 0.05, **p-value < 0.01, ****p-value < 0.0001. b Representative flow cytometry graphs of cellROX fluorescence intensity in NSCLC and cell lines in response to sertraline, irradiation, and the combination treatment.
