Fig. 5: Inflammation-related genes were downregulated in Med23-deleted mice.
From: Med23 deficiency reprograms the tumor microenvironment to promote lung tumorigenesis
a Heatmap analysis of differentially expressed genes between KrasG12D/+;Med23+/+ mice and KrasG12D/+;Med23f/f mice at 20 weeks post-infection. GO analysis of downregulated (b) and upregulated (c) genes (fold change > 2) in KrasG12D/+;Med23f/f mice. The black line indicates the gene counts and the blue bars indicate the statistics. d GSEA plot showing enrichment of antigen processing and presentation of a peptide antigen via MHC class I in KrasG12D/+;Med23+/+ mice. e The mRNA levels of B2m were confirmed by qRT‒PCR (n = 7 per group). f GO analysis of downregulated genes (fold change > 2) in MED23-knockdown A549 cells. The black line indicates the gene counts, and the blue bars indicate the statistics. g qRT‒PCR analysis of inflammation-related genes in lung tumors for both groups at 20 weeks post-Adeno Cre infection (n = 7 per group). h qRT‒PCR analysis of inflammation-related genes in A549 cells (n = 3 per group). i The mRNA level of B2M in A549 cells was analyzed by qRT‒PCR (n = 3 per group). The effect of Med23 knockdown (j) and Med23 overexpression (k) identified with a B2m promoter-luciferase reporter assay is shown (n = 3 per group). l The effect of overexpression of Med1 and Med24 on the B2m promoter-luciferase reporter assay is shown (n = 3 per group). Data are presented as the means ± SEMs. * P < 0.05, ** P < 0.01, *** P < 0.001.
