Fig. 4: The assessment of the cytotoxic capacity of pTcd8+cd28-.

Detection of expression of Perforin and Granzyme B in pTcd8+cd28- and pTcd8+cd28+ (a). Comparison of cell cluster composition between pTcd8+cd28- and pTcd8+cd28+ using single-cell RNA sequencing (b). Cell cluster annotation based on well-known cell markers (b, left). Cytotoxic score evaluation of annotated T-cell clusters (b, middle). Cell cluster distributions of pTcd8+cd28- and pTcd8+cd28+ (b, right); TCR repertoire comparison between pTcd8+cd28- and pTcd8+cd28+ (c). TCR clonality distribution in pTcd8+cd28- and pTcd8+cd28+ (c, upper). The shared clones between pTcd8+cd28- and pTcd8+cd28+ and clone expansion in pTcd8+cd28- (c, lower).