Fig. 2: Immune cell dynamics in a syngeneic mouse transplant model.

a A tumor transplant model was established by subcutaneous inoculation of the CT26 cell line into BALB/c mice. Control and mock samples were injected with PBS. b Growth curve of CT26 tumors in syngeneic BALB/c mice. The left y-axis indicates percentage of CD45⁺ Thy-1⁺ and CD45⁺ Thy-1^- cells in single cell suspension from tumor tissue. Tumor volume (right y-axis) was measured by Mean ± SEM (n = 10). c Cell type percentage graph illustrated an increase in lymphoid and myeloid cells during tumor growth. Frequency bar plots for subsets of lymphoid (d) or myeloid (e) cell types. Colors represent each subset. f UMAP plot of 2057 myeloid cells and their expression level of Il7r gene. The dot colors represent gene expression levels. g UMAP plot of representative macrophage feature genes including C1qa (resident macrophage), Cd14 (monocyte-like), and Il1b (pro-inflammatory) in myeloid cells. As shown in (f), the dot colors represent gene expression levels. h Immunofluorescence staining of CD127 (IL7R), CD68, and TNFα (left) or CD127, CD11c, and CXCL10 (right) in mouse tumors. Images were presented individually or were merged together (right bottom). Scale bar, 20 µm.