Fig. 6: The PRRX1–TOP2A interaction plays critical roles in migration and oncogenic signalling. | British Journal of Cancer

Fig. 6: The PRRX1–TOP2A interaction plays critical roles in migration and oncogenic signalling.

From: PRRX1-TOP2A interaction is a malignancy-promoting factor in human malignant peripheral nerve sheath tumours

Fig. 6

a Western blot analysis to detect 3xFLAG-PRRX1A and 3xHA-TOP2A. HS-PSS/3xFLAG-PRRX1A or HS-PSS/3xFLAG-PRRX1A/3xHA-TOP2A cells were treated with 1 µg/mL DOX for 1 day and then total cell lysates were extracted. b, c Comparison of migration capacity by wound healing assay. HS-PSS/3xFLAG-PRRX1A or HS-PSS/3xFLAG-PRRX1A/3xHA-TOP2A cells were treated with 1 µg/mL DOX for 1 day and then the assay was started (n = 6, three independent experiments). d Comparison of GSEA results performed on MSigDB for HS-PSS/3xHA-TOP2A, HS-PSS/3xFLAG-PRRX1A or HS-PSS/3xFLAG-PRRX1A/3xHA-TOP2A cells. In the significantly enriched oncogenic-related pathways, the size of the circle indicates the number of differentially expressed genes and the colour of the circle indicates the adjusted p value normalised by the z score. e Comparison of EMT-related genes (GREM1, ID2) mRNA among HS-PSS/3xHA-TOP2A, HS-PSS/3xFLAG-PRRX1A or HS-PSS/3xFLAG-PRRX1A/3xHA-TOP2A cells by qPCR analysis. Each cell was treated with 1 µg/mL DOX for 1 day and then the assay was started (n = 4, two independent experiments). All values were normalised to ACTB mRNA levels. Data are presented as the means ± SEMs. * p < 0.05; ** p < 0.01; *** p < 0.001.

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