Fig. 3: YAP1 exerts specific regulation over c-MET and CSC features through Ser397 phosphorylation.
a Western blot illustrating c-MET levels in the HCA46, SW48 and C10 cell lines. Tubulin was used as a loading control. Results are plotted as the average ± SD of all the biological replicates and were normalized to the HCA46 cell line (n = 3). b Colony formation assay of CRC cell lines HCA46, SW48 and C10.Results are plotted as the average ± SD of all the biological replicates. **p < 0.01 (n = 3), one-way ANOVA. c ALDH1 relative activity in CRC cell lines HCA46, SW48 and C10. Results were normalized to the cetuximab-sensitive HCA46 cell line. Results are plotted as the average ± SD of all the biological replicates. **p < 0.01, ***p < 0.001, one-way ANOVA. d Western blot illustrating c-MET expression in SW48 and C10 cell line transduced with YAP1 and YAP1S397A plasmids, along with the empty vector. Results were normalized to the YAP1-trasduced cell lines. e Colony formation assay of SW48 and C10 cell line transduced with YAP1 and YAP1S397A plasmids, along with the empty vector. Results are plotted as the average ± SD of all the biological replicates, **p < 0.01 (n = 3), one-way ANOVA. f ALDH1 relative activity in SW48 and C10 cell line transduced with YAP1 and YAP1S397A plasmids, along with the empty vector. Results were normalized to the empty vector condition in each cell line. Results are plotted as the average ± SD of all the biological replicates, *p < 0.05, **p < 0.01 (n = 3), one-way ANOVA. g SOX2 gene expression in SW48 and C10 cell line transduced with YAP1 and YAP1S397A plasmids, along with the empty vector. Results were normalized to the empty vector condition in each cell line. Results are plotted as the average ± SD of all the biological replicates *p < 0.05, **p < 0.01 (n = 3), one-way ANOVA.
