Fig. 5: Multiplexed single-cell imaging analysis of RSS.
From: Identification of unique rectal cancer-specific subtypes
a Highly multiplexed images (first row), virtual H&E images (second row), and cell type identifications (third row) of one of the RSS2 cancer tumours. All three TMAs belong to one patient, classified as RSS2. Colours of the multiplexed image: Blue-DAPI, Yellow-PCK26, and Red-GRP78. High GRP78 expression can be seen in epithelium tissues. b Boxplots of the immune cell percentages (first row) and expression levels of GRP78, β-catenin, CDX2 and Bcl-xl markers (Second and third row respectively). High expression of CDX2 in RSS3, GRP78 in RSS2, and low expression of β-catenin and Bcl-xl in RSS2 are observed at the single-cell level. Supporting the transcriptomics findings, higher Tregs infiltration is found in RSS1 and lower in RSS2. p-value annotations are as follows “**”p < 0.01; “*”p < 0.05; “ns” p > 0.05.
