Fig. 3: R54 reduced expression of PTEN and CD25 in PB-RCC Tregs.
From: Targeting CXCR4 impaired T regulatory function through PTEN in renal cancer patients
a PTEN and CD25 RNA expression in PB-Tregs treated for 30’ with R54 (10 µM) (Treg vs. Tregs+R54, p < 0.05). Histograms represent the mean ± sem (PTEN-RCC, n = 7; CD25-RCC n = 6 and PTEN-HD, n = 5; CD25-HD n = 6). b Flow cytometry CD25+PTEN+Tregs from RCC patients and HD. Isolated-Tregs were treated for 30’ with R54 (10 µM), washed and stimulated with CXCL12 (100 ng/mL) for 2’. In RCC (left): % CD25+PTEN+Tregs CXCL12 treated= 68 ± 3 vs. % CD25+PTEN+Tregs CXCL12 treated+R54 = 52 ± 6%, p < 0.05; (right): MFI PTEN+Tregs CXCL12 treated= 846 ± 60 vs. MFI PTEN+Tregs CXCL12 treated +R54 652 ± 59, p < 0.05. The box plot represents the median and spread of data within min to max value (RCC, n = 6; HD, n = 5). Paired and unpaired Student’s t- test was used. (*p < 0.05; **p < 0.01; ***p < 0.001). Data are derived from at least three independent experiments.
