Fig. 6: R54 decreased the expression of DNMT1 and FOXP3 in PB-RCC Tregs. | British Journal of Cancer

Fig. 6: R54 decreased the expression of DNMT1 and FOXP3 in PB-RCC Tregs.

From: Targeting CXCR4 impaired T regulatory function through PTEN in renal cancer patients

Fig. 6

Real-time PCR for isolated PB-RCC-Tregs (CD4+CD25+) and PB-HD-Tregs. RNA was extracted from frozen RCC and HD derived-Tregs pretreated with R54 (10 µM) for 30’(RCC: DNMT1 and FOXP3 Tregs vs. Tregs+R54, p < 0.05). Relative expressions of target genes were determined by the 2–ΔΔCt method using 18S and/or Beta2 microglobulin as endogenous control. Histograms represent the mean ± sem (CXCR4/DNMT1/TGF-β1-RCC, n = 12; FOXP3-RCC, n = 8; CXCR4/DNMT1/TGF-β1/FOXP3-HD, n = 6). A paired Student’s t-test was used. (*p < 0.05; **p < 0.01; ***p < 0.001). Data are derived from at least three independent experiments.

Back to article page