Fig. 3: VE-821, PF-477736 and MK-1775 sensitised HRP cells to rucaparib but not matched HRD cells. | British Journal of Cancer

Fig. 3: VE-821, PF-477736 and MK-1775 sensitised HRP cells to rucaparib but not matched HRD cells.

From: ATR, CHK1 and WEE1 inhibitors cause homologous recombination repair deficiency to induce synthetic lethality with PARP inhibitors

Fig. 3

a Single agent cytotoxicity of VE-821, PF-477736 and MK-1775 (24 h exposure) was measured by colony formation assay. Data are mean ± SEM of 3 independent experiments. LC50 values and further cell survival data are displayed in supplementary 1D. b–d Cells were exposed to rucaparib at indicated concentrations either as single agent or with the addition of 1 µM VE-821, 50 nM PF-477736 or 100 nM MK-1775 for 24 h prior to replacement with drug-free medium for 8-12 days to allow colony formation. Cell survival following exposure to combination treatments were normalised to single agent concentrations of VE-821 (1 µM), PF-477736 (50 nM) or MK-1775 (100 nM) in b Matched HRP and HRD cells c. C33A cells d SiHa cells and e IGROV-1 cells.

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