Fig. 2: Knockdown of PTGDS expression inhibited tumor growth of PTCL cells in vitro and in vivo. | British Journal of Cancer

Fig. 2: Knockdown of PTGDS expression inhibited tumor growth of PTCL cells in vitro and in vivo.

From: Targeting PTGDS Promotes ferroptosis in peripheral T cell lymphoma through regulating HMOX1-mediated iron metabolism

Fig. 2

a Western blotting was performed to verify the transfection efficiency. b Results of CCK8 assay at 48 h showed that PTGDS overexpression increased the proliferation of PTCL cells and PTGDS knockdown decreased it. c, d PTGDS knockdown inhibited the cell viability and the expression of c-myc in PTCL cells at 36 h. e–g Compared with sh-Control group, the growth rate, weight, volume and bioluminescence of tumor in mouse model were lower in sh-PTGDS group (n = 5 per group). h–j PTGDS knockdown induced cell cycle arrest at the G0/G1 phase, decreased the expression of CDK4, and increased the expression of CDK inhibitors P21 and P27 in PTCL cells at 36 h. k–m PTCL cells with PTGDS knockdown displayed higher apoptosis rate, increased expression of Bax and the cleaved forms of caspase-3, caspase-9 and PARP at 36 h. n–o PTGDS knockdown inhibited cell invasion and the expression of zeb1 and vimentin in PTCL cells at 36 h. p PTGDS knockdown regulated the expression of important proteins in tumor tissue from mouse model. Data are shown as the mean ± SD. *p < 0.05; **p < 0.01; ***p < 0.001.

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