Fig. 1: CTTNBP2 CRE SNV1 reduces the binding affinity between CTTNBP2 CRE and STAT3 which acts as transcriptional activator of CTTNBP2 gene.
From: Regulatory non-coding somatic mutations as drivers of neuroblastoma
Correlation between STAT3 and CTTNBP2 expression in a LAN-2 and b SH-SY5Y cells transfected with three pooled STAT3 siRNA. CTTNBP2 and STAT3 expression relative to their respective siScrambles (represented as single scramble bar in histogram), as measured by western blot (left) and qPCR (right) 24 h 48 h and 72 h post STAT3 silencing. β-Actin protein levels are used as loading control. c Luciferase reporter gene assay for CTTNBP2 CRE SNV1 carried out in LAN-2 and SH-SY5Y 72 h post STAT3 silencing. Luciferase activity of CTTNBP2 CRE SNV1 is normalized to that from cells transfected with wild-type construct and siScramble (scramble). d Allele-specific ChIP-qPCR conducted on plasmids carrying the wild-type or CTTNBP2 CRE SNV1 sequence upon transient transfection in LAN-2 and SH-SY5Y cell lines. Data is presented as fold-change of variant sequence upon comparing to wild-type sequence. All data shown are the mean ± standard deviation from three independent experiments each done in triplicate. Significant p-values obtained by two-tailed T-test are reported by * (* < 0.05; ** <0. 01; *** <0.001). Ref Reference, Alt Altered.
