Fig. 5: The deletion of the MCF2L CRE leads to decreased expression of MCF2L and increased cellular proliferation and invasion capacity. | British Journal of Cancer

Fig. 5: The deletion of the MCF2L CRE leads to decreased expression of MCF2L and increased cellular proliferation and invasion capacity.

From: Regulatory non-coding somatic mutations as drivers of neuroblastoma

Fig. 5

In edited clones, MCF2L expression relative to SK-N-BE(2) wild-type cells, as measured by western blot a and qPCR b. β-Actin protein levels are used as loading control. c Cell viability in edited clones is shown as fold change compared to SK-N-BE(2) wild-type cells. d Invading cells number in edited clones is shown as fold change compared to SK-N-BE(2) wild-type cells. Representative images (10×) of invasion assay in edited clones are reported on the right. All data shown are the mean ± standard deviation from three independent qPCR experiments, each done in triplicate. Significant p-values obtained by two-tailed T-test are reported by * (* < 0.05; ** < 0.01; *** < 0.001).

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