Fig. 4: Modification of the tumor immune microenvironment induced by combined NK-92/5.28.z cells and anti-PD-1 antibody treatment.
From: CAR-NK cell therapy combined with checkpoint inhibition induces an NKT cell response in glioblastoma
a–d Analyses of FFPE samples of orthotopic tumors using NanoString RNAseq data with comparison of differentially expressed genes after NK-92/5.28.z and anti-PD-1 combination therapy with all other treatment regimens in (a) Volcano plot and (b) Venn diagram. c Calculated cytotoxic and leukocyte cell scores using NanoString software. d Heatmap of differentially expressed genes associated with T cell pathways. e–g Flow cytometric analysis of explanted orthotopic tumors. e Cell counts of lymphoid and myeloid cell infiltration after treatment. f PD-L1-, PD-L2- and double-positive tumor cells after therapy. g PD-1-, Lag-3- and double-positive T cells after therapy.
