Fig. 5: Immunosuppressive-like TAMs are sensitive to ribosome-targeting therapy.

a Dotplot showing the comparison of pro/anti-inflammatory macrophage signature levels in each cluster (up), indicates pro/anti-inflammatory macrophage definition (bottom). b Heatmap showing the cellular ribosome biogenesis and pro/anti-inflammatory states grouped by tumor and tissue types within macrophages (Mφ). c Expression pattern of levels of pro/anti-inflammatory states and cellular ribosome biogenesis along pseudotime in the trajectory of macrophages. d Dotplot showing expressions of marker genes of pro- and anti-inflammatory macrophages in tumor murine models. Dot size indicates proportion of cells expressing genes, dot color indicates mean expression of genes. e Boxplots showing cellular ribosome biogenesis grouped by pro- and anti-inflammatory macrophages of tumor murine models. f Heatmaps showing tissue prevalence of pro- and anti-inflammatory macrophages estimated by Ro/e score grouped by CX-5461 pre- and post-treatment samples of tumor murine models. g Expression pattern of levels of Inflam/LA/Reg-TAM states and MRC1 along pseudotime in the trajectory of macrophages. h, i Multicolor-immunostaining of rRNA (Y10B), macrophages (CD68) and anti-inflammatory/LA/Reg-TAM states marker (MRC1) in tissues of CRC, LIHC, LUAD and STAD (h). Scale bar for all immunostaining images = 20 µm. ROI A represents MRC1⁺ TAMs, B represents MRC1^- TAMs, (i) showing the intensity of MRC1 and rRNA levels for each ROI in grouped by MRC1⁺ and MRC1^- TAMs. j Boxplots showing cellular ribosome biogenesis grouped by Mrc1⁺ Mφ and Mrc1^- Mφ of tumor murine models. k Boxplots showing cellular ribosome biogenesis in Mrc1⁺ Mφ and Mrc1^- Mφ of tumor murine models grouped by CX-5461 pre- and post-treatment samples. l Immunostaining of anti-inflammatory/LA/Reg-TAM states marker CD206 (red) and rRNA (Y10B, green) in TAMs upon CX-5461 treatment. Immunosuppressive-like TAMs obtained from conditioned medium treated RAW264.7 cells. Scale bar =100 µm.