Fig. 2: B7-H3 CAR-T cells target CRC in vitro. | British Journal of Cancer

Fig. 2: B7-H3 CAR-T cells target CRC in vitro.

From: The pronounced cytotoxic effects of chimeric antigen receptor T cells targeting B7-H3 in organoids and liver xenografts derived from colorectal cancer patients

Fig. 2

a Representative FCM plots of B7-H3 expression in different CRC cell lines. b, c CRC cells were co-cultured with CD19 or B7-H3 CAR-T cells for 72 h at an E:T ratio of 1:2, 1:1 or 2:1, respectively; subsequently, the enumeration of living CRC cells was performed using FCM. Representative FCM plots (b) and quantification of living tumor cells (c) are presented. Data are presented as mean ± SD. d–f The quantification of IFN-γ (d), IL-2 (e) and TNF-α (f) released by CAR-T cells in the supernatant of co-culture were measured by ELISA. Data are presented as mean ± SD. **p < 0.01.

Back to article page