Fig. 4: B7-H3 CAR-T cells target patient-derived CRC in intro and in vivo. | British Journal of Cancer

Fig. 4: B7-H3 CAR-T cells target patient-derived CRC in intro and in vivo.

From: The pronounced cytotoxic effects of chimeric antigen receptor T cells targeting B7-H3 in organoids and liver xenografts derived from colorectal cancer patients

Fig. 4

a, b Organoids of CRC were co-cultured with CD19 or B7-H3 CAR-T cells for 72 h at an E:T ratio of 1:2, 1:1 or 2:1, respectively; the representative immunofluorescence images (a) and quantification of residual living organoids (b) are illustrated. BF: bright field; green color, living organoids; red color, CAR-T cells. Scale bar=200 μm. Data are presented as mean ± SD. c Schema of the PDX liver metastatic model. d Representative ultrasound imaging of the PDX liver metastatic tumors pre- and after treatment with CD19 or B7-H3 CAR-T cells (upper panel), and the statistical results (bottom panel). Yellow triangle arrows show the margin of tumors. Scale bar = 2 mm. CR complete response. Data are presented as mean ± SD. e Mice livers and the PDX tumors inside after treatment with CD19 or B7-H3 CAR-T cells. PDX 1–3 represents 3 CRC patient donors. **p < 0.01.

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