Fig. 3: Differences in T-cell abundance in metastatic melanoma.

a Stacked bar plot representing the proportions of annotated CD4⁺ T-cell subsets (pink) and CD8⁺ T-cell subsets (green) and other identified immune subsets stratified by therapy response. b Dot plots of the median abundance of annotated CD4⁺ T-cells and CD8⁺ T-cells stratified for responder (R) and non-responder (NR). Each dot is a patient. The bars indicate the median with a 95% confidence interval. Mann-Whitney U-test was used to calculate the statistical difference between non-responders and responders. c Representative confetti images illustrating CD4⁺ T-cell subsets (pink) and various CD8⁺ T-cell subsets (green) in responders compared to non-responders. Scale bar is 40 μm. d Dot plots of the median abundancy of annotated CD8⁺ T-cells subsets including naive CD8⁺ T-cells, cytotoxic CD8⁺ T-cells (CD8⁺ CTL), regulatory CD8⁺ T-cells (CD8⁺ T_reg), CD8⁺ tissue resident T-cells (CD8⁺ T_RM), CD8⁺ memory T-cells (CD8⁺ T_MEM), CD8⁺ progenitor exhausted T-cells (CD8⁺ TPEX), and CD8⁺ terminally exhausted T-cells (CD8⁺ TEX), stratified for responder (R) and non-responder (NR). Each dot is a patient. The bars indicate the median with a 95% confidence interval. Mann-Whitney U-test was used to calculate the statistical difference between non-responders and responders. e Dot plots of the median abundancy of annotated CD4⁺ T-cells subsets, including naive CD4⁺ T-cells, cytotoxic CD4⁺ T-cells (CD4⁺ CTL), regulatory CD4⁺ T-cells (CD4⁺ T_reg), CD4⁺ memory T-cells (CD4⁺ T_MEM), CD4⁺ progenitor exhausted T-cells (CD4⁺ TPEX), and CD4⁺ terminally exhausted T-cells (CD4⁺ TEX), stratified by responder (R) and non-responder (NR). Each dot is a patient. The bars indicate the median with a 95% confidence interval. Mann-Whitney U-test was used to calculate the statistical difference between non-responders and responders. f Representative IMC images (left) of CD8⁺ TEX (indicated by TOX in magenta and CD8a in green), CD8⁺ TPEX (indicated by TCF1/7 in magenta and CD8a in green), CD8⁺ T_MEM (indicated by CD45RO in magenta and CD8a in green), and CD4⁺ CTL (indicated by Granzyme B in magenta and CD4 in green). Confetti images (right) illustrating CD4+ T-cell subsets (pink) and various CD8+ T-cell subsets (green). The scale bar is 40 μm.