Fig. 2: ExoView assay for measuring ALPPL2+ and THBS2+ exosome concentrations in serum samples.

a ExoView interferometric (IM) imaging size distribution of exosomes captured by antibodies against different tetraspanins in serum samples from a patient with PDAC. b Number of particles (exosomes) captured by antibodies against different tetraspanins in the serum sample from a patient with PDAC based on IM imaging. c Effects of detergent (Triton X-100) treatment on ExoView detection of ALPPL2+ and THBS2+ exosomes in serum samples from patients with PDAC. d Effects of permeabilization treatment on ExoView detection of ALPPL2+ and THBS2+ exosomes in serum samples from patients with PDAC. e Inter-assay CV (%) for the ExoView assays stratified by different tetraspanin captures. ****P < 0.0001; **P < 0.01. N ≥ 3 for each group.