Fig. 4: NETs induce chemoresistance in PDAC cells. | British Journal of Cancer

Fig. 4: NETs induce chemoresistance in PDAC cells.

From: Gemcitabine-induced neutrophil extracellular traps via interleukin-8-CXCR1/2 pathway promote chemoresistance in pancreatic cancer

Fig. 4

a Representative image after 72 h of incubation with each CM ± GEM administered to MIAPaCa-2 (scale bar: 200 μm). b Relative cell viability by XTT assay in MIAPaCa-2, KP4 and BxPC3. c Apoptosis assay after 72 h of incubation with each CM ± GEM administered to MIAPaCa-2. d A graph showing the ratio of increased apoptosis with GEM administration, with no GEM administration as the standard for each condition. e Relative cell viability by XTT assay in MIAPaCa-2 and KP4. PMA-NETs-mediated chemoresistance could not be reversed by DNase I or PAD4i. f DNA staining images after collecting MIA-NETs CM (scale bar: 100 μm). g Relative cell viability by XTT assay in MIAPaCa-2 and KP4. h Apoptosis assay after 72 h of incubation with each CM ± GEM administered to MIAPaCa-2. i A graph showing the ratio of increased apoptosis with GEM administration, compared to neither GEM or CM administration. *P < 0.05; **P < 0.01; ***P < 0.001. Data were presented as means ± SD.

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