Fig. 2
From: T-bet promotes potent antitumor activity of CD4+ CAR T cells
B7H6-specific CAR T cells expressing T-bet secrete different amounts of cytokine. a IFN-γ production by mouse CD4+ T cells (1 × 105) expressing the B7H6-specific CAR or the B7H6-specific CAR/T-bet co-cultured with RMA tumors (1 × 105), RMA-B7H6 (1 × 105), B16F10 tumors (2 × 104), or B16F10-B7H6 (2 × 104) for 24 h. Media was used as control. ANOVA Tukey test (***p < 0.001). Data shown are representative of three independent experiments. b Cell-free medium from CD4+ T cells expressing the B7H6-specific CAR with or without T-bet co-cultured with RMA-B7H6 for 24 h was analyzed for cytokine production. Student’s ttest (*p < 0.05, ***p < 0.001). Data shown are representative of two independent experiments
