Fig. 4

Clearance of recombinant mouse TFF2 and fusion TFF2-CTP-Flag from the blood. a–c Tff2-null mice were tail vein injected with equal molar amount of purified recombinant TFF2 and TFF2-CTP-Flag per kg of mouse weight. At indicated time points blood was taken and assayed for TFF2 and TFF2-CTP-Flag by western blot with antibodies produced against C-terminus of TFF2 molecule (a, b) and ELISA (c). Excretion of TFF2 with the urine was analyzed by western blot (a). ELISA data are plotted as a mean of value and standard deviation at each time point. d Dose-dependent downregulation of CCND1 mRNA in CD11b⁺Gr-1⁺ cells upon administration of recombinant wild-type TFF2 and fusion TFF2-CTP-Flag protein. Tff2-null mice were given 2.5% DSS for 5 days, then CD11b⁺Gr-1⁺ were sorted and cultured with recombinant TFF2 and TFF2-CTP-Flag in indicated concentrations for a 7 days