Fig. 1 | Cancer Gene Therapy

Fig. 1

From: Targeting GD2-positive glioblastoma by chimeric antigen receptor empowered mesenchymal progenitors

Fig. 1

MSCs are effectively transduced with GD2 tCAR and/or TRAIL encoding vectors. a Schematic presentation of the GD2 tCAR expression construct (upper) in which the IgM-derived anti-GD2 scFv was fused with the human CD8α-derived hinge-transmembrane domain and cloned between PGK and WPRE lentiviral regulatory elements, the mTRAIL expression construct (lower) carrying the full-length human TRAIL gene between PGK and WPRE. b FACS analysis of TRAIL and GD2 tCAR expression on empty vector-transduced MSCs, EV MSCs (row 1), GD2 tCAR MSCs transduced with GD2 tCAR vector (row 2), mTRAIL MSCs transduced with vector coding for mTRAIL (row 3), and bi-functional MSCs coding for both GD2 tCAR and mTRAIL (row 4)

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