Table 1 List of SNHG14 shRNA sequences and primer sequences for qRT-PCR were used in the study
Name | Primer | Sequence (5′-3′) |
|---|---|---|
shRNA1 | Sense | 5′-GCAAAUGAAAGCUACCAAU-3′ |
Anti-sense | 5′-AUUGGUAGCUUUCAUUUGC-3′ | |
shRNA2 | Sense | 5′-GCACAAUAUCUUUGAACUA-3′ |
Anti-sense | 5′-UAGUUCAAAGAUAUUGUGC-3′ | |
shRNA3 | Sense | 5′-CUAGAAUCCUAAAGGCAAA-3′ |
Anti-sense | 5′-UUUGCCUUUAGGAUUCUAG-3′ | |
SNHG14 | Forward | 5′-GGGTGTTTACGTAGACCAGAACC-3′ |
Reverse | 5′-CTTCCAAAAGCCTTCTGCCTTAG-3′ | |
GAPDH | Forward | 5′-AATTCCATGGCACCGTCAAG-3′ |
Reverse | 5′-TGGACTCCACGACGTACTC-3′ | |
IL-6 | Forward | 5′-AGTGAGGAACAAGCCAGAGC-3′ |
Reverse | 5′-AGCTGCGCAGAATGAGATGA -3′ | |
IL-1β | Forward | 5′-CAGAAGTACCTGAGCTCGCC-3′ |
Reverse | 5′-AGATTCGTAGCTGGATGCCG-3′ | |
TNF-α | Forward | 5′-GCTGCACTTTGGAGTGATCG-3′ |
Reverse | 5′-CTTGTCACTCGGGGTTCGAG-3′ | |
U6 | Forward | 5′-CTCGCTTCGGCAGCACA-3′ |
Reverse | 5′-AACGCTTCACGAATTTGCG-3′ | |
miR-136-5p | RT primer | 5′-CTCAACTGGTGTCGTGGAGTCGGCAAT TCAGTTGAGTCCATCA-3′ |
Forward | 5′-ACACTCCAGCTGGGACTCCATTTGTTTT-3′ | |
Reverse | 5′-CCAGTGCAGGGTCCGAGGT-3′ |
