Fig. 1

In vitro SGPL1 expression analysis. Evaluation of SGPL1 and SPHK1/2 isoenzyme transcript and protein expression in four RMS cell lines (RME: RD; RMA: RH-30, HA-OH1 and Ax-OH-1) and one rhabdoid cell line (A-204) compared to the non-tumorigenic primary human skeletal myoblasts (HSkM+ = differentiated; HSkM− = undifferentiated). Expression factors were calculated by densitometry and normalized to the HSkM control, which was set to 1. a RT-PCR of SGPL1, SPHK1, SPHK2 as well as controls: β-actin and GAPDH (loading control); myogenin and PAX3-FOXO1 (RMS/ myogenic marker); as well as ezrin and CXCR4 (metastasis marker). (Representative images of three independent experiments, n = 3). b Immuno blots of SGPL1, SPHK1, and SPHK2 as well as β-actin (loading control) and PCNA (proliferation control) (Representative example of three independent experiments, n = 3). c Network of SGPL1 protein interactions created with, https://string-db.org shows the evidence for co-expression of SGPL1 and SPHK2 (black line) and of SGPL1 interaction with SPHK1/2 (framed in red). All data and download files in STRING are freely available under a ‘Creative Commons BY 4.0’ license