Fig. 3
From: SGPL1321 mutation: one main trigger for invasiveness of pediatric alveolar rhabdomyosarcoma
Stimulation of RMA cells with S1P and DHS. a Metabolic cell viability was significantly increased in S1P stimulated (green bar) and significantly reduced in DHS stimulated (red bar) RH-30 and HA-OH1 cells compared to the vehicle control (0.1% MeOH). b The cell membrane impairment was measured by the release of the cytosolic lactate dehydrogenase (LDH). The vehicle control (0.1% MeOH) was set to 100%. Triton X-100 (T.X-100) treatment functioned as positive control. High DHS concentrations (10 µM) significantly increased (red bar) and the S1P stimulations slightly decreased (green bar) the extracellular LDH activity in supernatants of RH-30 and HA-OH1 cells. c Continuous treatment with 1 μM sphingosine-1-phosphate (S1P) significantly enhanced (red line) and 10 µM DHS treatment (green line) significantly reduced the migration speed compared to the vehicle control (0.1% MeOH) in RH-30 and HA-OH1 cells. Mean ± SD, n = 8 (MTS- and LDH-assay) and n = 4 (wound healing assay), *P < 0.05, **P < 0.01, ***P < 0.001, unpaired t-test
