Table 1 Overview and sequence of all used primer pairs for transcript amplification with RT-PCR
From: SGPL1321 mutation: one main trigger for invasiveness of pediatric alveolar rhabdomyosarcoma
Name | Forward Primer | Reverse Primer |
|---|---|---|
SGPL1 (Tv1) | 5′-ATGCCTAGCACAGACCTTCT-3′ | 5′-CTTCCTGGTGAGCTTAAAACA-3′ |
SGPL1 (Tv all) | 5′-ACTGCTCGCTTCCTCAAGTC-3′ | 5′-GTGACAGTGTCGGTGCTGTA-3′ |
GAPDH | 5′-CAAGGTCATCCATGACAACTTTG-3′ | 5′-GTCCACCACCCTGTTGCTGTAG-3′ |
β-Actin | 5′-GGGCATGGGTCAGAAGGATT-3′ | 5′-GAGGCGTACAGGGATAGCAC-3′ |
PAX3-FOXO1 | 5′-GCACTGTACACCAAAGCACG-3′ | 5′-CTGTGGATTGAGCATCCACC-3′ |
SPHK1 | 5′-TGGCGTCATGCATCTGTTCT-3′ | 5′-AGTAGTTTGGGTGCACCTGG-3′ |
SPHK2 | 5′-TCGTTCTGTGTCTGACCTGC-3′ | 5′-CATGAGCACAAAGTCCCCCT-3′ |
Ezrin | 5′-TGCGGAGCTTGCAGAATACA-3′ | 5′-GGATGCCCTCACTAGACAGC-3′ |
CXCR4 | 5′-TCCATTCCTTTGCCTCTTTTGC-3′ | 5′-CCAGACGCCAACATAGACCA-3′ |
Myogenin | 5′-GGTGCCCAGCGAATG-3′ | 5′-TGATGCTGTCCACGATCGA-3′ |
