Fig. 5
Downregulation of GATA3 in ccRCC tissues. a The total mRNA expression levels of GATA3 were examined by qRT-PCR in 30 pairs of normal and ccRCC tissues. Each dot in the graph represents one sample. *P < 0.05 vs. normal tissues. b Immunohistochemical analysis of GATA3 protein expression in ccRCC tissues and matched adjacent normal tissues. Higher staining of GATA3 was observed in normal renal tissues. c Whole protein was lysed from ten pairs of normal and ccRCC tissues. Western blot was performed to determine the relative protein level of GATA3. GAPDH was used as a control. The immunoblot shown is a representative image. N, paired normal tissue; T, tumor tissue. ***P < 0.001 vs. control. d The protein expression levels of GATA3 in HK-2, 768-O, 769-P, ACHN, and Caki-1 cells. **P < 0.005, ***P < 0.001 vs. HK-2 cells. Note that GATA3 was downregulated in the ccRCC tissues and cells
