Fig. 1: MyoD1 suppresses GC cell migration and invasion in vitro and in vivo. | Cancer Gene Therapy

Fig. 1: MyoD1 suppresses GC cell migration and invasion in vitro and in vivo.

From: MyoD1 suppresses cell migration and invasion by inhibiting FUT4 transcription in human gastric cancer cells

Fig. 1

a qRT-PCR and western blotting analysis of MyoD1 in SGC-7901 cells transfected with NC-siRNA and MyoD1 siRNA. The effects of knockdown of MyoD1 on SGC-7901 cell migration were determined by wound-healing assay at 0, 6, 12, 24, and 48 h (b) and Transwell assay (c). d qRT-PCR and western blotting analysis of MyoD1 in BGC-823 and MKN-45 cells transfected with empty vector and MyoD1 overexpression vector. e The effects of MyoD1 overexpression on GC cell migration were determined by wound-healing assay at 0, 6, 12, 24, and 48 h. The effects of MyoD1 overexpression on GC cell migration (f) and invasion (g) were determined by Transwell assay without or with Matrigel. h Western blotting analys×is of the levels of MMP-2, MMP-9, vimentin, and N-cadherin in MyoD1-overexpressed GC cell lines compared with the controls. i qRT-PCR and western blotting analysis of MyoD1 in MKN-45 cells transfected with lentiviral vector LV-MyoD1-MKN-45 and control (LV-CN). j Small animal imaging and metastasis-bearing lungs and other organs imaging analysis to assess tumor metastasis at day 21 after injecting. The significant differences were analyzed according to t-test (***p < 0.001; ****p < 0.0001).

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