Fig. 3: MyoD1 inhibits FUT4 transcription by binding its promoter. | Cancer Gene Therapy

Fig. 3: MyoD1 inhibits FUT4 transcription by binding its promoter.

From: MyoD1 suppresses cell migration and invasion by inhibiting FUT4 transcription in human gastric cancer cells

Fig. 3

a Relative expression levels of MyoD1 and FUT4 in LV-MyoD1-BGC-823 and LV-MyoD1-MKN-45 cells vs. control cells and in SGC-7901 cells in which MyoD1 was silenced MyoD1 expression was measured by qRT-PCR and western blotting. b Significant high expression of FUT4 mRNA in 415 GC vs. 35 normal gastric tissues according to the TCGA database (p < 0.001). c Higher expression of FUT4 in GC than in normal gastric tissues detected by IHC and according to the Human Protein Atlas. d Prediction of the promoter region of FUT4 and design of three pairs of primers (P1–P3) targeting fragments 1–3 (F1–F3) that covered full-length MBS of FUT4. e ChIP-qPCR of P1–P3 performed with anti-MyoD1 antibody. f Design of luciferase reporter for further validation of the direct binding by MyoD1 on MBS of FUT4 gene. Specific motifs in sequences are marked by yellow rectangles. g Luciferase activities for sequence 1, 2, and 3 were compared between pCMV2-GV146-MYOD1-transfected cells and pCMV2-GV146-transfected cells. The significant differences were analyzed according to t-test (*p < 0.05, **p < 0.01, ***p  < 0.001, ****p < 0.0001, n.s. not significant).

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