Fig. 4: MyoD1 overexpression alters UEA-I glycopattern binding in GC cells. | Cancer Gene Therapy

Fig. 4: MyoD1 overexpression alters UEA-I glycopattern binding in GC cells.

From: MyoD1 suppresses cell migration and invasion by inhibiting FUT4 transcription in human gastric cancer cells

Fig. 4

a FUT4 was mainly expressed in intracellular vesicles according to the Human Protein Atlas. b The glycopatterns of total glycoproteins from control and LV-MyoD1-MKN-45 cells defined by lectin microarrays. Fluorescent images were scanned at 70% photomultiplier tube and 100% laser power settings with a Genepix 4000B confocal scanner. A portion of the slide with three replicates of the lectin array is shown. The microarrays revealed increased normalized fluorescent intensities (NFIs, marked with red frames) and decreased NFIs (white frames) when LV-MyoD1-MKN-45 was compared with control MKN-45 cells. c NFIs and recognized glycans of lectins showed increased and decreased signals in LV-MyoD1-MKN-45 vs. control MKN-45 cells from lectin microarray (ratio >1.5 or < 0.67); the significant differences were analyzed according to t test (*p < 0.05, **p < 0.01, ***p < 0.001). d Lectin immunofluorescence approach to validate and investigate lectin binding sites in controls and LV-MyoD1-BGC-823 and LV-MyoD1-MKN-45 cells. The images were acquired with Cy3, DAPI, and merge channels (×120 objective magnification).

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