Table 1 All primers sequences and oligonucleotide sequences.
Primer name | Sequence | |
|---|---|---|
Primers for qRT-PCR | ||
β-Actin F | CCAACCGCGAGAAGATGA | |
β-Actin R | CCAGAGGCGTACAGGGATAG | |
MyoD1-F | AGCACTACAGCGGCGACT | |
MyoD1-R | GCGACTCAGAAGGCACGTC | |
FUT4-F | AAGGTCCAGGCCCACTGAAG | |
FUT4-R | CAGTTCAGGTGACAGAGGCTCAA | |
Oligonucleotide sequences for siRNA | ||
Negative siRNA (NC-siRNA) sense | UUCUCCGAACGUGUCACGUTT | |
Negative siRNA (NC-siRNA) antisense | ACGUGACACGUUCGGAGAATT | |
MYOD1 siRNA sense | GUAAAUGAGGCCUUUGAGATT | |
MYOD1 siRNA antisense | UCUCAAAGGCCUCAUUUACTT | |
FUT4 siRNA-1 sense | CAUGUGACCGUGGACGUGUTT | |
FUT4 siRNA-1 antisense | ACACGUCCACGGUCACAUGTT | |
FUT4 siRNA-2 sense | CUCGCAGCACCUGGAUUAUTT | |
FUT4 siRNA-2 antisense | AUAAUCCAGGUGCUGCGCGAGTT | |
Primers for ChIP | ||
Primer 1 | ||
Primer 1-F | CTGTCTTTACACCCTTAACTTGG | |
Primer 1-R | TATTGCCTGTTCAGTGAGATG | |
Primer 2 | ||
Primer 2-F | TTATGAGTCCTGTGCGTCTT | |
Primer 2-R | CCAGCCCAGTAAAGGATAGA | |
Primer 3 | ||
Primer 3-F | GGGCTGGCAGGCAAAGGAAA | |
Primer 3-R | CCCGCCGAGACGGTCGAATT | |
