Fig. 3: miR-34c-3p overexpression increases apoptosis in KRAS-mutated NSCLC cells.
From: miR-34c-3p targets CDK1 a synthetic lethality partner of KRAS in non-small cell lung cancer
a- Annexin-V and propidium iodide staining of HCC827 cells simultaneously transfected with miR-34c-3p and KRAS mutant vector for 48 h. Cells transfected with scrambled miRNA and an empty vector were used as control. The graph on the right reports the quantification (analysed by FlowJo) of the number of live cells and apoptotic cells in each experimental point. Two-way Anova was used for comparison (*p ≤ 0.05; **p ≤ 0.01). b Annexin-V and propidium iodide staining of PT#18 cells co-transfected with miR-34c-3p and KRAS mutant vector. Data were analysed with the BD AccuriTM C6 plugin software. ANOVA was used for comparison (*p ≤ 0.05; **p ≤ 0.01). Experiments were performed twice.
