Fig. 4: Overexpression of miR-34c-3p induces caspases activation in KRAS-mutated NSCLC cells.
From: miR-34c-3p targets CDK1 a synthetic lethality partner of KRAS in non-small cell lung cancer
a Caspase-3 activation measurement in HCC827, A549, and primary epithelial NSCLC cell lines (PT #2 and PT #13) upon 48 h of transfection with miR-34c-3p and KRAS mutant vector. Bar graphs indicate mean value ± SD and the p value was calculated by ANOVA test. Experiments were performed in technical duplicate and repeated three times. b FACS analysis of caspase-3 activation of in HCC827 cells upon simultaneous transfection of miR-34c-3p and KRAS mutant vector. Cells transfected with an empty vector and a scrambled miRNA were used as control. Bar graphs indicate mean value ± SD and the p-value was calculated by ANOVA test. Experiments were performed twice. c Caspase-3 activation measured in HCC827 and primary epithelial NSCLC cell lines (PT #2 and PT #13) upon anti-miR-34c-3p transfection (an anti-scrambled miRNA was used as control). Experiments were performed in technical duplicate and repeated three times. Bar graphs indicate mean value ± SD and the p-value was calculated by ANOVA (*p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001;****p ≤ 0.0001).
