Fig. 1: Diagrammatic representation of the acellular lung (ACL) model.

Right lung lobes were tied by silk, and 2D cells were seeded to the left lung lobe through the trachea. As the nutrient media perfuse to the lung, tumor nodules on left lobes grow and form dCTCs that intravasate to the vasculature, survive in circulation, and enter the right lobes through the pulmonary artery (PA) and forms metastatic lesions.