Fig. 4: The expression of EMT-associated TFs suggests that OS-D dCTCs may have undergone dedifferentiation. | Cancer Gene Therapy

Fig. 4: The expression of EMT-associated TFs suggests that OS-D dCTCs may have undergone dedifferentiation.

From: Transcriptional activators YAP/TAZ and AXL orchestrate dedifferentiation, cell fate, and metastasis in human osteosarcoma

Fig. 4

A As assessed by RT-PCR, dCTCs derived from the ex vivo 4D ACL model have significantly higher SOX9, TWIST, and ZEB1 gene expression, as compared to respective primary tumor nodules formed on this model and OS-D cells grown as 2D monolayer. Columns represent the mean values of n = 3 experiments, and bars represent standard deviations. B Representative SOX9, TWIST, and ZEB1 immunofluorescence confocal microscopic staining and quantification, C within the single cell, or D the averaged OS-D-2D, PT, and dCTC samples. 20 μm scale bars are shown. Scatter plots represent the mean value of three experiments for OS-D 2D-monolayer cultures and four experiments for the PT and dCTC. Bars represent standard deviations. PT primary tumor, dCTC derived circulating tumor cell.

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