Fig. 4: Effect of JNK KD on cell proliferation and colony formation. | Cancer Gene Therapy

Fig. 4: Effect of JNK KD on cell proliferation and colony formation.

From: c-Jun N-terminal kinase 2 suppresses pancreatic cancer growth and invasion and is opposed by c-Jun N-terminal kinase 1

Fig. 4

A, B Effect of JNK down-regulation on basal cell proliferation. Indicated cells (10,000 cells/well for MIA PaCa-2, 5000 cells/well for PANC-1) were cultured for 48 h in 96-well plates in complete medium and analyzed by the MTT assay. Results are shown as optical density and are means (±SD) from three separate experiments with quadruplicate determinations. C–F Anchorage-independent growth. MIA PaCa-2 was not influenced by JNK downregulation (C). JNK2 KD in PANC-1 increased colony formation and size while JNK1 KD showed opposing effects (D, E). Representative images of PANC-1 colony size (left, 2.5× magnification) and colony formation (right, scanned image after MTT staining) are shown in (F). Scale bar: 1 mm. Data are shown as means of three independent experiments of triplicate determinations ±SD. JNK WT cells (WT and Neo clones), JNK1 KD clones, and JNK2 KD clones were analyzed together. ***p < 0.001 compared to JNK WT cells.

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