Fig. 1: Multiple myeloma cell lines express CD46 and are killed by LOAd viruses.
From: Immunostimulatory oncolytic virotherapy for multiple myeloma targeting 4-1BB and/or CD40
Human multiple myeloma cell lines ANBL-6, L363, LP-1, OPM-2, RPMI-8226, and U266-84 were analyzed for their expression of CD46 by flow cytometry. Gray filled histograms represent matched isotype controls and black lines represent CD46 staining (a). All abovementioned cells lines were infected with LOAd viruses or replication-deficient Ad5/35 Mock virus at different virus to cell ratios (10, 20, 50, and 200 MOI). Cell viability was analyzed with MTS viability assay at 72 h post infection and viability is shown as percentage viability of uninfected control cells. n = 2; technical replicates = 4; graphs show mean ± SEM.
